WebThe “chromatin interaction analysis by paired-end tag sequencing” (ChIA-PET) is an extension of Hi-C in the sense that ChIA-PET selects for ligation products of interactions … Webpaired-end tag (plural paired-end tags) Any of the short sequences, at the 5’ and 3’ ends of a DNA fragment, that are sufficiently unique (theoretically) so as to exist together only once …
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WebApr 30, 2012 · The binding of transcription factors to specific regulatory sequences brings about a network of transcription regulation and coordination (1, 14). Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) was developed to identify these higher-order chromatin structures (5,6). Paired-end tags (PET) (sometimes "Paired-End diTags", or simply "ditags") are the short sequences at the 5’ and 3' ends of a DNA fragment which are unique enough that they (theoretically) exist together only once in a genome, therefore making the sequence of the DNA in between them available upon search (if … See more PET libraries are typically prepared in two general methods: cloning based and cloning-free based. Cloning based Fragmented genomic DNA or complementary DNA … See more 1. DNA-PET: Because PET represent connectivity between the tags, the use of PET in genome re-sequencing has advantages over the … See more pelicans roost condominiums sanibel fl
Paired-End Tag - an overview ScienceDirect Topics
WebShare. ChIA-PET (Chromatin Interaction Analysis by Paired-End Tag Sequencing) was introduced in 2009 and combines ChIP with chromatin conformation capture (3C) … WebThe 3K Long-Tag Paired End library DNA fragments comprise an approximately 250-bp fragment with a 44-mer adaptor sequence in the middle, flanked by 100-mer sequences, on average. Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET or ChIA-PETS) is a technique that incorporates chromatin immunoprecipitation (ChIP)-based enrichment, chromatin proximity ligation, Paired-End Tags, and High-throughput sequencing to determine de novo long-range chromatin interactions genome-wide. Genes can be regulated by regions far from the promoter such as regulatory elements, insulators … mechanical engineering current events