Witryna13 mar 2007 · The 2-iminothiolane reaction with protein amino groups adds a spacer arm ending with a thiol group, which can be further treated with molecules carrying a maleimido ring. This approach is currently used for the preparation of a candidate ‘blood substitute’ in which human Hb (haemoglobin) is conjugated with long chains of PEG … WitrynaReaction with proteins is performed at pH 7-10 in aqueous buffer. RNA-protein conjugation is performed by reaction with 2-iminothiolane followed by a mild ultraviolet irradiation treatment (Wover 1983 for ribosomal subunits) Literature PubMed: Traut RR, Bollen A, Sun TT, Hershey JW, Sundberg J, Pierce LR.; Methyl 4 …
Formation of non-amidine products in the reaction of primary …
WitrynaThe reagent 2-iminothiolane (2-IT) is used to introduce thiol groups into proteins and peptides by reactions of their amino groups. In this study, we report that the thiol adduct initially formed ... Witryna1 sie 2016 · The reaction can be monitored by 2-iminothiolane’s absorbance at 248 nm. ... The reaction mixture was kept at 23 °C for 1 h and the excess reagent was removed by dialysis against the same buffer solution at 4 °C. The sample was dialyzed with this buffer overnight, and then dialyzed with PBS buffer pH 6.6 for 1 h. ... sql query to check tde enabled or not
2-Iminothiolane = 98 TLC, powder 4781-83-3
2-Iminothiolane reacts with primary amines efficiently at pH 7 to 9, creating amidine compounds with a sulfhydryl group. Thus it allows for crosslinking or labeling of molecules such as proteins through use of disulfide or thioether conjugation. It was first used to thiolate a subunit of ribosome in E. coli in 1973 by Robert Traut, its namesake, and his colleagues. WitrynaFigure Legend Snippet: a Survival of EcN after reaction with 2-iminothiolane for varied time intervals, including 60, 90, 120, and 150 min. b Survival, c growth curves, and d surface thiol numbers of EcN after reaction with different concentrations of 2-iminothiolane ranging from 0 to 400 µg/ml for 90 min. e Zeta potentials of EcN after … Witryna1 lut 2009 · Influence of the reaction time and amounts of 2-iminothiolane on the transferrin thiolation (n = 3). The following amounts were used: 25.4-, 50.85-, 76.2- … sql query to check oracle version