Circrna pulldown
WebFeb 1, 2024 · Importantly, using circRNA pulldown assay and RNA-binding protein immunoprecipitation, we identified PABPC1 as a robust novel interacting protein of circPTK2. Mechanistically, circPTK2 could bind to PABPC1 and enhance its ability to stabilize SETDB1 mRNA, thereby specifically promoting SETDB1 expression and … WebcircRNA可被包裹成外泌体，参与细胞间信号传递，促进多种肿瘤的恶性进展。CD8+T细胞功能障碍是肝细胞癌（HCC）免疫逃逸的主要因素。然而，外泌体来源的环状RNA …
Circrna pulldown
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WebCircRNA pull-down assay. A biotin-labeled circPTPRA probe was designed and synthesized by RiboBio Co., Ltd. The RNA pull-down assay was performed according to the instruction of the Pierce™ Magnetic RNA–Protein Pull-Down Kit (Thermo Scientific). The probe sequences used for the RNA pull-down assay are summarized in Additional … WebJan 15, 2024 · Biotin-coupled miRNA and circRNA pull-down assays were performed as described previously [11, 12]. Briefly, 3′ end biotinylated circRNA-ACAP2 (digoxin-5′-TCACACAGGCAGCTTTGGAAGAAG-3′-Digoxin; RiboBio, Guangzhou, China) was transfected into SW480 cells at a final concentration of 20 nM for 1 day. Biotin-coupled …
WebJan 4, 2024 · The oncogenic roles of circRTN4 in PDAC tumor growth and metastasis were studied in mouse tumor models. Bioinformatics analysis, luciferase assay and miRNA pulldown assay were performed to study the novel circRTN4-miRNA-lncRNA pathway. To identify circRTN4-interacting proteins, we performed circRNA-pulldown and mass … WebFeb 15, 2024 · We designed biotin probes for circTUBGCP4 targeting the back splicing junction and verified the good pull-down efficiency in HUVECs transfected overexpression circTUBGCP4 (Fig. 6c). The circRNA pull-down results showed that the circTUBGCP4 probe could significantly bind miR-146b-3p, not miR-873-5p in the HUVEC lysis buffer …
WebOct 5, 2024 · CircRNA are known as micro RNA (miRNA) sponges due to their ability to bind to miRNA molecules and influence their function. They play a key role in the … WebJun 2, 2024 · A novel and highly specific circRNA-pulldown followed by mass spectrometry analysis identified 94 circFOXK2-interacting proteins, which were involved in cell adhesion, mRNA splicing, and structural molecule activity. Of these, circFOKX2 interactions with YBX1 and hnRNPK enhanced expression of oncogenes NUF2 and PDXK.
WebFeb 15, 2024 · Identification of the circRNA-binding proteins can be achieved by circRNA-pulldown method [33,34,35]. Molecular mechanisms and functions of circular RNA Accumulated evidence has provided a focus ...
WebApr 7, 2024 · RNA pull down -MS/WB 筛选或验证目标RNA互作基因或蛋白，研究相应的分子调控机制。 3. ChIP-seq 筛选或验证目标蛋白与DNA互作，研究相应的分子调控机制。 云序生物服务优势 优势一：云序累计支持客户发表 100 + 篇RNA修饰SCI论文，合计影响因子 960 + 优势二：累计完成数千例 RNA甲基化测序样本，全面覆盖医口、农口等各类样本。 … origins of aaveWebMar 23, 2024 · 6.2.1. CircRNA Pull-Down and RIP. CircRNA pull-down is the most efficient way to detect the interaction between circRNA and its binding proteins. To date, … origins of 1st world warWebcircRNA Pull-down Assay IntegrateRNA is an outstanding biotechnology company with years of experience in RNA-protein interaction analysis. We offer customized circRNA … how to wrap a swaddle photographyWebFig 1. Schematic diagram of circRNA functioning as miRNA/RBP sponge. Creative Biogene emlpoys dual luciferase reporter assay system to validate the sponge function of … origins of ab bloodWebAug 21, 2024 · The proteins associated with the circRNA can be studied using methods such as mass spectrometry and Western blotting, while the RNAs present in the … origins of ability groupingWebDec 1, 2024 · CircRNA-centric methods to study circRNA-protein interactions3.1. Antisense-based pulldown purification of circRNPs. The principle of antisense … how to wrap a tailgateWeb微信公众号外泌体之家介绍：外泌体（exosome）及细胞外膜泡（EVs）领域核心平台，为外泌体研究的同行们提供最新最专业的科研资讯；播报最新精准医学前沿资讯，服务广大生命科学及医学工作者和大众。；国内外泌体领域进展总结（2024年3月） how to wrap a stroller for baby shower